Wet mounting is a direct microscopic examination of feacal specimens.
AIM: To detect the presence of motile parasites, white blood cells (WBC), red blood cell (RBC), casts, cysts, and eggs of parasites, as an aid in the diagnosis of helminthic infections.
MATERIALS/APPARATUS: Stool specimen, binocular microscope, glass slides, cover slip, bulb pipette physiological/normal saline, applicator stick, cotton wool, discard jar.
- Place 2 drops of normal saline on a clean glass slide using a bulb pipette.
- Using the applicator stick, collect a small amount of the feaces and mix it with the normal saline on the glass slide.
- As you emulsify/mix the feacal specimen and the normal saline together, make sure that a thin smooth preparation is made. Otherwise, the preparation will be too thick, making it difficult to detect and identify parasites or any of its components present in the specimen.
- Cover the preparation/mixture on the glass slide with a cover slip.
- Examine the prepared slide under the microscope using ×10 objective lens to focus, and then ×40 objective lens to confirm.
Note: The condenser iris of the microscope should be closed sufficiently before viewing in order to get a very good contrast. Also, always make sure that you examine several fields of the slide before making your final report, so as to clear all doubts.
REPORTING OF THE RESULT:
Report the appearance of the stool specimen first based on its colour and consistency. Report the number of larvae and egg/ova found as: scanty, few, moderate number, many, or very many. The presence of WBC, RBC, or cysts is also reported using + sign, and if not found in the specimen; they are reported as “nil”.