AIM: To isolate pathogenic bacteria from urogenital specimens (e.g. vaginal swab, urethral swab) as an aid in the diagnosis of Candidiasis and bacterial vaginosis or bacterial infections of the ureter, vagina, and cervix.
MATERIAL/APPARATUS: Urogenital specimen, blood agar (BA), cystein lactose – electrolyte deficient (CLED) agar, incubator, chocolate agar, grease pencil, Bunsen burner, inoculating loop.
- Label the culture plates (CLED, BA, and CA) with the patient’s name and hospital number using the grease pencil.
- Aseptically inoculate the urogenital specimen on the CLED, BA, and CA plates.
- Incubate the CLED and BA plates in the incubator at 37oC overnight.
- Incubate the CA plate in an anaerobic jar at 37oC overnight.
- Perform biochemical testing on significant bacterial growth.
- Perform antimicrobial susceptibility testing on significant bacterial isolates.
REPORTING OF THE RESULT:
Report only significant bacterial growth after performing biochemical tests to identify the isolated organisms. Bacteria likely to be isolated from urogenital specimens include: Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli,, Proteus, Enterococcus, Neisseria gonorrhoeae. N. gonorrhoeae is isolated from urogenital specimens using a selective enriched culture medium media called Thayer Martin medium.