General Microbiology

Techniques of enumerating bacterial cell numbers and cell mass

Written by MicroDok

Many techniques exist for the measurement of microbial growth in terms of assessing microbial cell numbers and microbial cell mass. These methods as stipulated in Table 1 below helps the microbiologists to elucidate the doubling (generation) time of a microbial cell. As explained in Table 1, these techniques also allow the microbiologist to assess the actual amount or number of both viable and dead cells present in the growth medium. The type of measurement technique to be employed is usually dependent on the type of analysis the microbiologist seeks to undertake.

Table 1: Techniques of enumerating bacterial cell numbers and cell mass


Brooks G.F., Butel J.S and Morse S.A (2004). Medical Microbiology, 23rd edition. McGraw Hill Publishers. USA. Pp. 248-260.

Madigan M.T., Martinko J.M., Dunlap P.V and Clark D.P (2009). Brock Biology of microorganisms. 12th edition. Pearson Benjamin Cummings Publishers. USA. Pp.795-796.

Prescott L.M., Harley J.P and Klein D.A (2005). Microbiology. 6th ed. McGraw Hill Publishers, USA. Pp. 296-299.

Ryan K, Ray C.G, Ahmed N, Drew W.L and Plorde J (2010). Sherris Medical Microbiology. Fifth edition. McGraw-Hill Publishers, USA.

Singleton P and Sainsbury D (1995). Dictionary of microbiology and molecular biology, 3rd ed. New York: John Wiley and Sons.

Talaro, Kathleen P (2005). Foundations in Microbiology. 5th edition. McGraw-Hill Companies Inc., New York, USA.

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