Gene Therapy Basics

SYNOPSIS OF HOW GENE THERAPY WORKS

Written by MicroDok

Genes are made of deoxyribonucleic acid (DNA) molecules – which are the main genetic material of living cells. It is in the gene that the genetic information that directs the activities of a cell is located; and this genetic information also direct the production of malfunctioned proteins or activities that result in genetic disorder in a living host. Successful gene delivery requires an efficient way to get the DNA into living host cells; and to make it happen, a vector (usually a virus) is used to deliver the gene of interest into the host receiving the gene therapy. These DNA delivery vehicles or systems are known generally as vectors. Vectors are cloning agents that act as self-replicating DNA molecules, and which is used in molecular biology techniques to carry cloned genes or other segments of DNA into another recipient host.

It is noteworthy to note that in parasitology, a vector may mean an agent which is usually an insect or animal that is able to carry infectious disease agents or pathogens from one host to another. To be successful in gene therapy techniques, a vector must be able to target the right type of cells in the recipient host. It must be able to integrate the gene or DNA it is carrying into the host cell. Vectors must leave no untoward effect on the recipient host and the vector must also be able to activate the gene once it has entered the host cell. The inserted desired gene must go to the nucleus of the target host cell and be “turned on. For a gene to be turned on, it means that the gene is transcribed and translated (according to the central dogma of molecular biology as aforesaid) in order to make the desired protein product that the inserted gene encodes.

And for the gene delivery process to be successful, the protein must function properly in the recipient host cell. Gene therapy as aforementioned is the addition of new genes to a patient’s cells to replace missing or malfunctioning genes – as a panacea to correcting a particular molecular/genetic disease or disorder. It is usually done using a virus to carry the genetic cargo into cells, because that’s what viruses evolved to do with their own genetic material. A vector which can be viral or nonviral vector carrying the desired gene or DNA is used to deliver the functional/normal gene into the recipient patient’s target cell or tissues. The vector carrying the therapeutic gene (viral vector in particular) infects and invades the non-functional tissue or cell, and transforms it. Particularly, the viral vector (harbouring the therapeutic DNA) uncoats and releases the therapeutic gene into the host cell genome. Taking control of the infected host cell’s genome, the therapeutic gene starts to synthesize functional or normal genes which bring about the desired effect by replacing the non-functional gene product or repairing the defective gene; and this returns the diseased target cell to its normal physiological function.

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