AIM: To isolate pathogenic bacteria from semen specimens as an aid in the diagnosis of infertility in male.
MATERIAL/APPARATUS: Semen specimen, grease pencil, incubator, anaerobic jar, Bunsen burner, inoculating loop, blood agar (BA), chocolate agar (CA).
- Label the CA and BA plates with the patient’s name and laboratory number using the grease pencil.
- Aseptically culture the semen specimen on both the CA and BA plates.
- Incubate the BA plate in the incubator at 37oC overnight.
- Incubate the CA plate in the anaerobic jar at 37oC overnight.
- Perform biochemical test to identify the isolated bacteria.
- Perform antimicrobial sensitivity test only when a significant bacteria is isolated.
REPORTING OF THE RESULT:
Significant bacteria growth should be reported. The culture of semen specimen is carried out first before any microscopical analysis in order to avoid contaminating the specimen.