Microbiology Laboratory

SEMINAL FLUID (SEMEN) CULTURE

Written by MicroDok

AIM: To isolate pathogenic bacteria from semen specimens as an aid in the diagnosis of infertility in male.

MATERIAL/APPARATUS: Semen specimen, grease pencil, incubator, anaerobic jar, Bunsen burner, inoculating loop, blood agar (BA), chocolate agar (CA).

METHOD/PROCEDURE:

  1. Label the CA and BA plates with the patient’s name and laboratory number using the grease pencil.
  2. Aseptically culture the semen specimen on both the CA and BA plates.
  3. Incubate the BA plate in the incubator at 37oC overnight.
  4. Incubate the CA plate in the anaerobic jar at 37oC overnight.
  5. Perform biochemical test to identify the isolated bacteria.
  6. Perform antimicrobial sensitivity test only when a significant bacteria is isolated.

REPORTING OF THE RESULT:

Significant bacteria growth should be reported. The culture of semen specimen is carried out first before any microscopical analysis in order to avoid contaminating the specimen.

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MicroDok

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