Cell / Tissue Culture

REASONS FOR DOING CELL CULTURE

Written by MicroDok

Why is cell, tissue or organ culture embarked upon despite the ethical issues surrounding its development, acceptance and usage? Cell culture has application in a variety of biomedical sciences and even in the industry. It has become one of the major tools used in the life sciences world over. Its application has greatly revolutionized the field of medicine, biotechnology and even agriculture owing to the many useful products derived from it. Cells are cultured for so many reasons. Some of these reasons are highlighted in this section.

  • Cell culture is an important source of pharmaceuticals such as vaccines, proteins, antibodies, hormones and even the anti-diabetic hormone called insulin.
  • It helps to study the metabolism of living cells including cells of microbes, plants and animals.
  • Cell culture helps to provide evidence on the function and development of specific cells.
  • It is used for the isolation and growth of cancerous cells for further studies.
  • Cell culture gives a clue as to how cancerous cells develop and spread.
  • It can be used for the screening of putative drugs in the pharmaceutical industry during novel drug development.
  • It is a tool for investigating the numerous processes relating to human health and diseases.
  • Cell culture is used to test new products including drugs, cosmetics, vaccines and other pharmaceuticals for toxicity.

REFERENCES

Lindee, S.M.  2007.  The Culture of Cell Culture.  Science; 316(5831): 1568-1569

Lodish H, Berk A, Matsudaira P, Kaiser C.A, Kreiger M, Scott M.P, Zipursky S.L and Darnell J (2004). Molecular Cell Biology. Fifth edition. Scientific American Books, Freeman, New York, USA.

Marcovic O and Marcovic N (1998). Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cell Dev Biol. 34:108.

Mather J and Barnes D (1998). Animal cell culture methods, Methods in cell biology. 2rd eds, Academic press, San Diego.

Fleming, D. O., Richardson, J. H., Tulis, J. I. and Vesley, D. (eds) (1995). Laboratory Safety: Principles and practice. Washington DC: ASM press.

Freshney R.I (2005). Culture of Animal Cells, a Manual of Basic Technique, 5th Ed. Hoboken NJ, John Wiley and Sons Publishers.

Health Services Advisory Committee (HSAC) (2003). Safe Working and the Prevention of Infection in Clinical Laboratories. HSE Books: Sudbury

Caputo J.L (1996). Safety Procedures. In: Freshney, R.I., Freshney, M.G., eds., Culture of Immortalized Cells. New York, Wiley-Liss, Pp. 25-51.

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MicroDok

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