Exam Questions / Microbiology


Written by MicroDok
  1. A boy was born with a rare genetic defect that resulted in adenosine deaminase (ADA) deficiency. After 10 years, the child’s immune system still remained weakened. ADA is required for the synthesis of functional B and T cells – which are both critical for a functional immune system. The doctors recommended the replacement of the defective gene with a functional one in order to restore the synthesis of ADA in the boy. As an intending molecular biologist, which aspect of genetic engineering is best suitable to handle this medical condition? Define this aspect of generic engineering briefly; and mention the two (2) types of it.


  1. Polymerase chain reaction (PCR) technique is a molecular biology tool used for the amplification of a particular gene of interest. Elucidate briefly the 3 important steps of this technique while taking note of their varying temperature conditions.


  1. Define the following terms: Gene; Plasmid; Genome; Restriction enzyme; Proteomics.


  1. Enumerate and explain briefly any (4) areas in which genetic engineering can be applied in the practice of agriculture.


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