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- What is cell culture and passaging?
- Describe the different type of cell culture techniques.
- Explain how cells for cell culture techniques can be obtained or procured.
- Explain the term cell line with examples.
- The hood is critical for cell culture techniques. Explain.
- What are the merits and demerits of cell culture techniques?
- Explain the use of antibiotics in some cell culture techniques.
- Mention the ways of preventing contamination during cell culture experiments.
- X-ray the continued use of animals for biomedical/medical research despite the availability of cell culture techniques.
- Explain the 3R’s guiding the use of animals for biomedical research.
- Define and explain the phrases: explants, explantation and cytotoxicity?
- X-ray the different physicochemical environmental factors that affect cell culture techniques.
- Enumerate the reasons for performing cell culture.
- Succinctly describe HeLa
- Enumerate the different ways of obtaining cell lines for carrying out cell culture technique.
- What is the meaning of the acronym “CHO” as it relates to cell lines?
- Highlight on the importance of the hood or biosafety cabinet in cell culture technique.
- Why are inverted microscopes preferred in cell culture techniques to other types of microscopes?
- Explain the function of haemocytometer and centrifuge in cell culture techniques.
- Write a short note on the application of cell culture in any areas of your choice.
- What is vivisection?
- What does the acronym RPMI and DMEM stand for?
- Enumerate the components of RPMI 1640 medium.
- Outline the procedure for preparing cell culture medium.
- What are the reasons for checking cells under the microscope.
- Enumerate the procedure of resuscitating a frozen cell culture line
