• What is cell culture and passaging?
• Describe the different type of cell culture techniques.
• Explain how cells for cell culture techniques can be obtained or procured.
• Explain the term cell line with examples.
• The hood is critical for cell culture techniques. Explain.
• What are the merits and demerits of cell culture techniques?
• Explain the use of antibiotics in some cell culture techniques.
• Mention the ways of preventing contamination during cell culture experiments.
• X-ray the continued use of animals for biomedical/medical research despite the availability of cell culture techniques.
• Explain the 3R’s guiding the use of animals for biomedical research.
• Define and explain the phrases: explants, explantation and cytotoxicity?
• X-ray the different physicochemical environmental factors that affect cell culture techniques.
• Enumerate the reasons for performing cell culture.
• Succinctly describe HeLa
• Enumerate the different ways of obtaining cell lines for carrying out cell culture technique.
• What is the meaning of the acronym “CHO” as it relates to cell lines?
• Highlight on the importance of the hood or biosafety cabinet in cell culture technique.
• Why are inverted microscopes preferred in cell culture techniques to other types of microscopes?
• Explain the function of haemocytometer and centrifuge in cell culture techniques.
• Write a short note on the application of cell culture in any areas of your choice.
• What is vivisection?
• What does the acronym RPMI and DMEM stand for?
• Enumerate the components of RPMI 1640 medium.
• Outline the procedure for preparing cell culture medium.
• What are the reasons for checking cells under the microscope.

Enumerate the procedure of resuscitating a frozen cell culture line