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- Define “growth” in the context of microorganisms.
- What is binary fission?
- Define doubling time.
- What is batch culture?
- What is a continuous culture?
- Briefly explain a chemostat.
- Define turbidity.
- Explain in details the microbial growth curve.
- Enumerate four methods of determining microbial cell numbers.
- What is the significance of spectrophotometer in determining microbial cell mass?
- Differentiate between viable cell count and total cell count.
- What is optical density (OD)?
- Diagrammatically illustrate the growth curve of bacteria.
- How can microbial cell mass be enumerated?
- Mathematically illustrate the formular for calculating optical density (OD).
- What is turbidometry and spectrophotometry?
- Which instrument is routinely used to measure viable cell numbers of microbes in samples?
- How can viral cells be enumerated in a sample?
- When is a bacterium defined as “dead”?
- What are the basic parameters measured and used to determine microbial growth.
