Microbiology Laboratory

DIFFERENTIAL COUNT OF CSF SAMPLE

Written by MicroDok

AIM: To provide information on the different white blood cells (neutrophils, basophils, eosinophils, lymphocytes, monocytes) present in the CSF specimen.

MATERIAL/APPARATUS: CSF specimen, methylene blue stain, microscope, glass slide, water, Bunsen burner, inoculating loop, immersion oil.

METHOD/PROCEDURE:

  1. Place a drop of the CSF specimen on a clean glass slide.
  2. Spread using a sterilized inoculating loop and allow to dry.
  3. Heat fix the smear on the glass slide.
  4. Flood the slide with methylene blue stain.
  5. Allow for 5 minutes. Then wash off the methylene blue stain with water.
  6. Allow the slide to dry completely.
  7. Add a drop of immersion oil on the slide.
  8. View the slide under the microscope using the ×100/oil immersion objective lens.
  9. Systematically examine the slide and count the different white cells seen in each field.

REPORTING OF THE RESULT:

Report the number of the different white cells seen.

NOTE: Differential white cell count can be performed using 3 types of stains:

  • Methylene blue stain.
  • Giemsa stain.
  • Leishma stain

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MicroDok

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