Cell / Tissue Culture

Cytotoxicity

Written by MicroDok

Cytotoxicity is the cellular damage of metabolic pathways, structures and intracellular processes of a living organism which ultimately result in the loss of function or impaired metabolic function. In some cases, cytotoxicity may lead to the loss of viability of the lining cells or tissues. Most in vitro experimentations (particularly cell culture techniques) are mainly aimed at determining the probable toxicity or cytotoxicity of substances (e.g. drugs, cosmetics and vaccines) that is being tested using cell culture techniques. And because these tested materials or substances are used in vivo by living organisms (inclusive of humans) for treatment and other beneficial purposes, it is critical that they are certified safe to the host’s body and thus portend no danger or toxicity when used. Cytotoxicity testing is used to determine the level of toxicity of a substance at the cellular level either in vivo or in vitro. It can be carried out in vivo (i.e. in laboratory animals) or in vitro (as is applicable with cell culture). Cytotoxicity testing is crucial for the testing of products such as drugs and vaccines as aforementioned before they are released into the market for public consumption.

References

Freshney R.I (2005). Culture of Animal Cells, a Manual of Basic Technique, 5th Ed. Hoboken NJ, John Wiley and Sons Publishers.

Health Services Advisory Committee (HSAC) (2003). Safe Working and the Prevention of Infection in Clinical Laboratories. HSE Books: Sudbury

Caputo J.L (1996). Safety Procedures. In: Freshney, R.I., Freshney, M.G., eds., Culture of Immortalized Cells. New York, Wiley-Liss, Pp. 25-51.

Cooper G.M and Hausman R.E (2004). The cell: A Molecular Approach. Third edition. ASM Press.

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MicroDok

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