Microbiology Laboratory

CULTURING OF FUNGAL SPECIMENS 

Written by MicroDok

AIM: To isolate and identify pathogenic fungi in patient’s specimens as an aid in the diagnosis of fungal infections (mycoses)

MATERIAL/APPARATUS: Patient’s specimen, incubator, inoculating loop, Bunsen burner, S+C tube, S+C+A tube, lactophenol cotton blue reagent,  glass slide, cover slip, microscope.

S = Sabouraud’s dextrose agar (SDA)

C = Chloramphenicol

A = Actidine (cyclohexamide)

METHOD/PROCEDURE:

  1. Label the S+C and S+C+A tubes with the patient’s biodata (e.g. name, date of culturing, laboratory number, and type of specimen).
  2. Collect the specimen using a sterilized inoculating loop.
  3. Inoculate the specimen on the slope and butt of the S+C and S+C+A tubes.
  4. Incubate the tubes in the incubator at 28oC for about 21 days.
  5. Check the incubated tubes for fungal growth at different time intervals before the 21 days elapses.
  6. After the 21 days, bring out the S+C and S+C+A tubes from the incubator.
  7. Make wet preparations of the fungal growths in both tubes on a clean glass slide using the lactophenol cotton blue stain.
  8. Cover the prepared slide with a cover slip and view with the ×10 and ×40 objective lens of the microscope.

REPORTING OF THE RESULT:

The name of the fungus responsible for the disease condition of the patient is reported after a thorough microscopical investigation is carried out. Familiarity with fungal elements and structures also helps in this process.

About the author

MicroDok

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