Cell / Tissue Culture

BASIS FOR A SUCCESSFUL CELL CULTURE

Written by MicroDok

Having a cell culture without any form of contamination is paramount in the cell culture laboratory. Such success is usually achieved when the laid down principles and aseptic techniques for undertaking a cell culture procedure are conscientiously followed. A successful cell culture technique depends on a number of factors. These factors are highlighted in this section.

  • The quality of cell lines you are working with.
  • The quality of reagents and cell culture media used.
  • The aseptic technique used.
  • The quality of the laboratory equipment used and their operation.
  • The experience of the researcher.

When these factors are met and made available prior to undertaking cell culture experimentation, it is expected that the cell culture technique will be successful.

References

Lodish H, Berk A, Matsudaira P, Kaiser C.A, Kreiger M, Scott M.P, Zipursky S.L and Darnell J (2004). Molecular Cell Biology. Fifth edition. Scientific American Books, Freeman, New York, USA.

Marcovic O and Marcovic N (1998). Cell cross-contamination in cell cultures: the silent and neglected danger. In Vitro Cell Dev Biol. 34:108.

Mather J and Barnes D (1998). Animal cell culture methods, Methods in cell biology. 2rd eds, Academic press, San Diego.

Fleming, D. O., Richardson, J. H., Tulis, J. I. and Vesley, D. (eds) (1995). Laboratory Safety: Principles and practice. Washington DC: ASM press.

Freshney R.I (2005). Culture of Animal Cells, a Manual of Basic Technique, 5th Ed. Hoboken NJ, John Wiley and Sons Publishers.

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MicroDok

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