Having a cell culture without any form of contamination is paramount in the cell culture laboratory. Such success is usually achieved when the laid down principles and aseptic techniques for undertaking a cell culture procedure are conscientiously followed. A successful cell culture technique depends on a number of factors. These factors are highlighted in this section.
- The quality of cell lines you are working with.
- The quality of reagents and cell culture media used.
- The aseptic technique used.
- The quality of the laboratory equipment used and their operation.
- The experience of the researcher.
When these factors are met and made available prior to undertaking cell culture experimentation, it is expected that the cell culture technique will be successful.
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